Development of a Pulsed-Field Gel Electrophoresis (PFGE) method for molecular typing of clinical isolates of Arcanobacterium haemolyticum期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Development of a Pulsed-Field Gel Electrophoresis (PFGE) method for molecular typing of clinical isolates of Arcanobacterium haemolyticum

Authors:	Flores Aurea E Diedrick Michelle J Ferrieri Patricia

Institution:	^a Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA ^b Department of Pediatrics, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA

Abstract:	We developed a two-block PFGE method to study molecular variation among clinical isolates of Arcanobacterium haemolyticum, an often overlooked human pathogen. Three main macrorestriction profiles were defined among 15 isolates. PFGE was an objective method for characterizing A. haemolyticum and may be useful in molecular epidemiological studies of this organism.

Keywords:	Arcanobacterium haemolyticum Pulsed-Field Gel Electrophoresis (PFGE) Macrorestriction profiles Molecular variation Molecular typing
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