Development of an extended multilocus sequence typing for genotyping of Brucella isolates期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Development of an extended multilocus sequence typing for genotyping of Brucella isolates

Authors:	Chen Yanfen Zhen Qing Wang Yufei Xu Jie Sun Yingjie Li Tiefeng Gao Lina Guo Fei Wang Dali Yuan Xitong Yuan Jing Huang Liuyu Chen Zeliang Yu Yaqin

Affiliation:	^a School of Public Health, Key Laboratory of Zoonosis, Ministry of Education, Jilin University, Changchun, Jilin 130021, PR China ^b Department of Infectious Disease Control, Beijing Institute of Disease Control and Prevention, Beijing, 100071, PR China ^c Songyuan Center for Disease Control and Prevention, Songyuan, Jilin 138000, PR China ^d First Institute of Endemic Disease Control of Jilin Province, Baicheng, Jilin 137000, PR China

Abstract:	By amplifying and sequencing longer sequences, an extended multi locus sequence typing (EMLST) theme was developed for Brucella. 61 isolates were genotyped by the EMLST with increased resolution. This strategy could be extended to other bacteria to improve MLST genotyping resolution without additional loci.

Keywords:	Brucella Multi locus sequence typing Genotyping
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