Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua

Authors:	Løvdal Trond Hovda Maria Befring Björkblom Benny Møller Simon G

Institution:	^a Nofima Norconserv AS, Box 8034, N-4068 Stavanger, Norway ^b Centre for Organelle Research, Faculty of Science and Technology, University of Stavanger, 4036 Stavanger, Norway ^c The Norwegian Centre for Movement Disorders, Stavanger University Hospital, 4068 Stavanger, Norway

Abstract:	The combination of propidium monoazide (PMA) and quantitative real-time PCR (qPCR) significantly overestimated the fraction of viable Listeria innocua as compared to plate counts and confocal fluorescence microscopy. Our data imply that PMA-qPCR must be used with caution as an analytical tool for the differentiation between viable and dead bacteria.

Keywords:	Listeria innocua Propidium monoazide Quantitative real-time PCR Viability
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