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Fluorescence in situ hybridization for phytoplasma and endophytic bacteria localization in plant tissues
Authors:Bulgari Daniela  Casati Paola  Faoro Franco
Affiliation:
  • a Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Via Celoria 2, 20133 Milano, Italy
  • b CNR, Istituto di Virologia Vegetale, Strada delle Cacce 73, 10135 Torino, Italy
  • Abstract:In the present study, we developed a rapid and efficient fluorescence in situ hybridization assay (FISH) in non-embedded tissues of the model plant Catharanthus roseus for co-localizing phytoplasmas and endophytic bacteria, opening new perspectives for studying the interaction between these microorganisms.
    Keywords:Video-confocal microscope   DNA probe   Vibroslice   Periwinkle
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