Survey of bromodeoxyuridine uptake among environmental bacteria and variation in uptake rates in a taxonomically diverse set of bacterial isolates |
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| Authors: | Hellman Maria Berg Jeanette Brandt Kristian K Hallin Sara |
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| Institution: | a Swedish University of Agricultural Sciences, Department of Microbiology, Box 7025, SE-750 07 Uppsala, Swedenb Department of Agriculture and Ecology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark |
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| Abstract: | Incorporation of 5-Bromo-2′-Deoxyuridine (BrdU) into DNA can be used to target replicating bacteria in the environment, but differential uptake capacity is a potential bias. Among 23 bacterial isolates commonly found in soils, most took up BrdU, but at up to 10-fold different cell-specific rates. Combined with results from an in silico analysis of 1000 BrdU-labeled 16S rRNA gene sequences, our results demonstrate a BrdU uptake bias with no apparent relationship between taxa affiliation and ability to incorporate BrdU. |
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| Keywords: | Active bacteria BrdU incorporation Replicating bacteria Soil bacteria Thymidine analog |
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