An atomic force microscopy method for the detection of binding forces between bacteria and a lipid bilayer containing higher order gangliosides |
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Authors: | Adams Elizabeth L Almagro-Moreno Salvador Boyd E Fidelma |
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Affiliation: | a Delaware Biotechnology Institute, University of Delaware, Newark, DE, USAb Department of Biological Sciences, University of Delaware, Newark, DE, USA |
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Abstract: | We developed an atomic force microscopy (AFM) method to determine the binding forces between a model cell wall plasma membrane and Vibrio cholerae. V. cholerae cells were covalently attached to AFM probes and forces were determined against a lipid bilayer containing sialic acid (N-acetylneuraminic acid) molecules as well as several control surfaces. |
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Keywords: | AFM, atomic force microscope APTES, 3-aminopropylmethyldiethoxysilane SAM, self assembled monolayer PBS, phosphate buffered saline HBS, HEPES buffered saline POPC lipids, palmitoyl-oleyl-sn-phosphatidyl-choline |
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