Development of a URA5 integrative cassette for gene disruption in the Candida guilliermondii ATCC 6260 strain期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Development of a URA5 integrative cassette for gene disruption in the Candida guilliermondii ATCC 6260 strain

Authors:	Millerioux Yoann Clastre Marc Simkin Andrew J Marais Emeline Sibirny Andriy A Noël Thierry Crèche Joël Giglioli-Guivarc'h Nathalie Papon Nicolas

Affiliation:	^a EA2106, Biomolécules et Biotechnologies Végétales, Faculté de Pharmacie, Université François Rabelais, Tours, France ^b Institute of Cell Biology, NAS of Ukraine, Drahomanov Street, 14/16, Lviv, 79005, Ukraine ^c University of Rzeszow, Department of Biotechnology and Microbiology, Cwiklinskiej, 2, Rzeszow 35-601, Poland ^d UMR 5234 CNRS Université Bordeaux 2, Microbiologie Cellulaire et Moléculaire et Pathogénicité, Département 2 Parasitologie et Mycologie Moléculaire, Bordeaux, France

Abstract:	We designed an efficient transformation system for Candida guilliermondii based on a ura5 ATCC 6260 derived recipient strain and a URA5 recyclable selection marker. This “URA5 blaster” disruption system represents a powerful tool to study the function of a large pallet of genes in this yeast of clinical and biotechnological interest.

Keywords:	Transformation system Selectable marker Orotate phosphoribosyltransferase Candida guilliermondii
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