Genotyping of Brazilian Erysipelothrix spp. strains by amplified fragment length polymorphism |
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| Authors: | Coutinho Tania Alen Imada Yumiko de Barcellos David Emílio Santos Neves de Oliveira Sérgio José Moreno Andrea Micke |
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| Institution: | a Laboratório de Sanidade Suína e Virologia, Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Universidade de São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, CEP 05508 270, São Paulo/SP, Brazilb Department of Biologicals Production, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japanc Setor de Suínos, Faculdade de Veterinária (FAVET), Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9090, Agronomia, CEP 91540-000, Porto Alegre, Brazild Laboratório de Microbiologia Clínica e de Alimentos, Faculdade de Veterinária, Universidade Luterana do Brasil (ULBRA), Av. Farroupilha, 8001, São José, CEP 92425-900, Canoas, Brazile Laboratório de Sanidade Suína e Virologia - FMVZ/USP, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, CEP 05508 270, São Paulo/ SP, Brazil |
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| Abstract: | One hundred and fifty-one Erysipelothrix spp. isolates from diseased and carrier swine from Brazil were identified by PCR, submitted to serotyping and analyzed by amplified fragment length polymorphism with a single enzyme (AFLP).Reference strains from Australia and the United Kingdom were also examined. The 151 strains were classified into 18 different serotypes (1a, 1b, 2a, 2b, 4, 5, 6, 7, 8, 10, 11, 12, 15, 17, 19, 21, 24 and 25), being serotype 2b the most frequent (39.7%). By associating serotyping and PCR results, it was possible to identify 146 strains as E. rhusiopathiae and five strains as E. tonsillarum. Despite the fact that for this genus AFLP did not cluster all isolates according to serotype, origin, disease or isolation data, the execution of the technique was easy and fast, demonstrating high discriminatory power. The results produced by the AFLP analysis of Erysipelothrix spp. could also support its use as a discriminatory tool for E. rhusiopathiae and E. tonsillarum species. |
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| Keywords: | AFLP Erysipelothrix spp Genotyping Serotyping Swine |
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