Bacterial classification using genomic fingerprints obtained by virtual hybridization |
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Authors: | Jaimes-Díaz Hueman García-Chéquer Adda Jeanette Méndez-Tenorio Alfonso Santiago-Hernández Juan Carlos Maldonado-Rodríguez Rogelio Beattie Kenneth Loren |
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Affiliation: | a Laboratory of Biotechnology and Genomic Bioinformatics, Department of Biochemistry, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México City, Mexicob Amerigenics Inc, Crossville, TN, USA |
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Abstract: | In silico genomic fingerprints were produced by virtual hybridization of 191 fully sequenced bacterial genomes using a set of 15,264 13-mer probes specially designed to produce universal whole genome fingerprints. A novel approach for constructing phylogenetic trees, based on comparative analysis of genomic fingerprints, was developed. The resultant bacterial phylogenetic tree had strong similarities to those produced from the alignment of conserved sequences. Notably, the trees derived from the alignment of other conserved COG genes divided the Bacillus and Corynebacterium genera into the same subgroups produced by the novel bacterial tree. A number of discrepancies between both techniques were observed for the grouping of some Lactobacillus species. However, a detailed analysis of the alignment of these genomes using other bioinformatics tools revealed that the grouping of these organisms in the novel tree was more satisfactory than the groupings from previous classifications, which used only a few conserved genes. All these data suggest that the bacterial taxonomy produced by genomic fingerprints is satisfactory, but sometimes different from classical taxonomies. Discrepancies probably arise because the fingerprinting technique analyzes genomic sequences and reveals more information than previously used approaches. |
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Keywords: | Genomic fingerprints Virtual hybridization Bacterial identification DNA arrays |
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