Sensitive counting of viable Enterobacteriaceae in seawaters and relationship with fecal indicators期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Sensitive counting of viable Enterobacteriaceae in seawaters and relationship with fecal indicators

Authors:	Baudart J Robyns A Peuchet S Drocourt J L Lebaron P

Affiliation:	^a UPMC Univ Paris 06, UMR 7621, LOMIC, Observatoire Océanologique, F-66650, Banyuls/mer, France ^b CNRS, UMR 7621, LOMIC, Observatoire Océanologique, F-66650, Banyuls/mer, France ^c AES-CHEMUNEX, R&D department, F-94854, Ivry/seine, France

Abstract:	A fluorescent in situ hybridization based assay was used to enumerate viable Enterobacteriaceae members in seawaters by solid phase cytometry. The method was specific, highly sensitive (1 cell/100 ml) and allowed the quantification of VNC Enterobacteriaceae cells during an osmotic stress. Investigations on contaminated coastal seawater revealed a strong correlation between Enterobacteriaceae counts and standard fecal indicators.

Keywords:	Enterobacteriaceae Viable cell Solid phase cytometer
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