PCR-based community structure studies of bacteria associated with eukaryotic organisms: a simple PCR strategy to avoid co-amplification of eukaryotic DNA期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

PCR-based community structure studies of bacteria associated with eukaryotic organisms: a simple PCR strategy to avoid co-amplification of eukaryotic DNA

Authors:	Bakke Ingrid De Schryver Peter Boon Nico Vadstein Olav

Affiliation:	^a Norwegian University of Science and Technology, Department of Biotechnology, Sem Saelands v. 6/8, 7491 Trondheim, Norway ^b Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, B-9000 Gent, Belgium

Abstract:	PCR primers targeting conserved regions of the SSU rRNA gene are commonly used in bacterial community studies. For microbes associated with eukaryotes, co-amplification of eukaryotic DNA may preclude the analysis. We present a simple and efficient PCR strategy to obtain pure bacterial rDNA amplicons from samples predominated by eukaryotic DNA.

Keywords:	Bacterial SSU rDNA Eukaryotic SSU rDNA Co-amplification Nested PCR Biased PCR DGGE
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