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NUCLEOTIDE SEQUENCE HOMOLOGY OF SOME ALGAL RIBOSOMAL RNAs1
Authors:Guat Lean Soh  Gary Kochert
Abstract:Radioactively labeled ribosomal RNA from seven species of algae was purified and hybridized to purified DNA from Chlamydomonas reinhardtii Dangeard (Chlorophyta). Delta Tm values for the heterologous RNA-DNA duplexes were obtained by thermal dissociation; values ranged from 3.9 to 10.9 C. Organisms traditionally considered to be less closely related to C. reinhardtii showed the greatest delta Tm values. Ulothrix fimbriata Bold and Klebsormidium flaccidum (Kütz.) S., M. & Bl., although similar morphologically, showed very different delta Tm values and would not appear to be closely related by these criteria.
Keywords:Chlamydomonas  Cryptomonas  DNA  Dunaliella  electrophoresis  Klebsormidium  Navicula  nucleic acid hybridization  phylogeny, algal  ribosomal RNA  Scenedesmus  Volvox
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