Plant regeneration of Mangifera indica using liquid shaker culture to reduce phenolic exudation |
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| Authors: | S. S. Raghuvanshi Alka Srivastava |
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| Affiliation: | (1) In vitro culture and Plant Genetics Unit, Department of Botany, University of Lucknow, (226007) Lucknow, India |
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| Abstract: | The exudation of phenolics from the cut ends of mango explants greatly hinders their regenerative ability in any in vitro growth medium. However, pretreatment of explants using liquid shaker culture helps in overcoming this problem. Explants kept in liquid MS medium supplemented with 1% polyvinylpyrolidone in 250 ml conical flasks on an automated shaker at 75 rpm were able to produce shoots when inoculated on gelled MS medium supplemented with different concentrations of growth regulators.Abbreviations BA benzyladenine - IAA indoleacetic acid - NAA naphthaleneacetic acid - IBA indolebutyric acid |
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| Keywords: | in vitro culture micropropagation mango |
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