PCR analysis of Actinobacillus actinomycetemcomitans,Porphyromonas gingivalis,Treponema denticola andFusobacterium nucleatum in middle ear effusion |
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| Institution: | 1. Department of Microbiology, Faculty of Dentistry, Faculty of Medicine, Istanbul University, Istanbul;2. Faculty of Dentistry, Department of Microbiology, Faculty of Medicine, Istanbul University, Istanbul;3. Department of Microbiology, Faculty of Medicine, Karadeniz Technical University, Trabzon;4. Department of Otorhinolaryngology, Head and Neck Surgery, Taksim Training and Research Hospital, Istanbul;5. Department of Oral Implantology, Faculty of Dentistry, Istanbul University, Istanbul;1. School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia;2. Bioanalytical Mass Spectrometry Facility, University of New South Wales, Sydney, New South Wales, Australia;3. Centre for Infectious Diseases and Microbiology–Public Health, Institute of Clinical Pathology and Medical Research – Pathology West, Westmead Hospital, New South Wales, Australia;4. Marie Bashir Institute for Infectious Diseases and Biosecurity, Sydney Medical School, University of Sydney, New South Wales, Australia;1. Oral Biosciences, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Sai Ying Pun, Hong Kong SAR, China;2. Oral Rehabilitation, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Sai Ying Pun, Hong Kong SAR, China;3. Department of Oral and Maxillofacial Implantology, Shanghai Ninth People''s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China;4. Universities of Bern and Zurich, Schools of Dental Medicine, Switzerland;5. Oral Diagnosis and Polyclinics, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Sai Ying Pun, Hong Kong SAR, China;1. Department of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, CA, 92093-0116, USA;2. Institute of Microbiology, University of Agriculture, Faisalabad, Punjab, Pakistan;3. Department of Basic and Translational Sciences, Penn Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104;4. Research Center for Advanced Oral Science, Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-8514, Japan;5. Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104;6. Institute for Clinical Chemistry and Laboratory Medicine, Faculty of Medicine, Technische Universität Dresden, 001069 Dresden, Germany;1. Department of Orthodontics Science, Kanagawa Dental University, Yokosuka, Japan;2. Department of Microbiology, Kanagawa Dental University, Yokosuka, Japan |
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| Abstract: | Anaerobes contribute to the severity and chronicity of infections that occur in and around the oral cavity. One of the factors involved in the pathogenesis of otitis media with effusion (OME) is the retrograde movement of bacteria from the oropharynx into the middle ear cavity. OME is one of the most common causes of hearing loss in children. We have used a PCR-based method to identify Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola andFusobacterium nucleatum in 65 middle ear effusion (MEE) samples obtained from paediatric patients seen for myringotomy and tube placement. DNA was extracted from MEE samples and PCR was initially done with DNA extracts by using the universal primers within the 16S rRNA gene sequence common to all bacterial species. The positive samples were further assessed with four species-specific primers. With the universal primers, 27 of 65 samples (41.5%) showed positive reaction indicating the presence of bacterial DNA. F. nucleatum was present in 10 out of 27 PCR-positive samples (37%) while one sample was positive for both T. denticola and F. nucleatum (3.7%). A. actinomycetemcomitans and P. gingivalis were not detected in any of the samples. The results of this study suggest that oral bacterial species may also play a role in the aetiopathogenesis of paediatric MEE. |
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