The Response Regulator FimR Is Essential for Fimbrial Production of the Oral Anaerobe Porphyromonas gingivalis |
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| Institution: | 1. Department of Microbiology, School of Dentistry, Aichi-Gakuin University, Nagoya, 464-8650, Japan;2. Scientific Frontier Promoted Research Center, School of Dentistry, Aichi-Gakuin University, Nagoya, 464-8650, Japan;1. Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea;2. Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea;3. Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea;4. Division of Restorative Dentistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Canada;6. Department of Conservative Dentistry, School of Dentistry, Seoul National University, Seoul 03080, Republic of Korea;1. Department of Stomatology, Nanfang Hospital and College of Stomatology, Southern Medical University, Guangzhou, China;2. Center of Oral Implantology, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, China;3. Department of Stomatology, Maoming People''s Hospital, Maoming, China;4. Department of endodontics, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, China;1. Department of Periodontology, Operative and Preventive Dentistry, Bonn University, Dental Faculty, 53111 Bonn, Germany;2. Clinical Study Support Core, University Hospital Bonn, 53015 Bonn, Germany;3. Center of Applied Medical Laser Research and Biomedical Optics (AMLaReBO), Bonn University, 53111, Germany;1. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China;2. Key Laboratory of Biotechnology and Bioresources Utilization (Dalian Minzu University), Ministry of Education, Dalian, Liaoning, 116600, China;3. College of Food Science and Technology, Bohai University, Food Safety Key Lab of Liaoning Province, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou, Liaoning, 121013, China;1. Department of Oral Microbiology and Immunology, and Dental Research Institute, School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea;2. Department of Agricultural Biotechnology, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea;3. Department of Oral Histology and Developmental Biology, and Dental Research Institute, School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea |
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| Abstract: | Porphyromonas gingivalis is a Gram-negative oral anaerobe considered to be a major pathogen in adult periodontitis. One of the noted virulence factors of this bacterium is its unique fimbriae, which are composed of FimA (fimbrilin) as a major subunit. We have recently identified and isolated two essential genes, fimS and fimR, for fimbriation of P. gingivalis from transposon-mutagenesis studies. The genes encode two components of a His–Asp phosphorelay system, FimS as a sensor histidine kinase and FimR as a response regulator. Disruption of either gene causes fimbrial deficiency in this organism. In this study, the expression of FimR protein was detected in various P. gingivalis strains. In addition, a fragment containing fimR with a possible promoter was introduced into the fimR -disruption mutant, using a shuttle vector, pT-COW. The transconjugant recovered both FimR and FimA expression at levels comparable to the parentP. gingivalis ATCC 33277. Furthermore, characteristic fimbrial structures were clearly observed around the cell surface of both parent and transconjugant cells under electron microscopy. This is the first successful complementation experiment in P. gingivalis. These results show that the FimR protein is essential as a positive regulator in fimbriation of P. gingivalis. |
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