不同条件对植物中间纤维体外装配的影响(简报)

细胞骨架的研究是当今细胞生物学中最为活跃的领域之一,而中间纤维是三种主要骨架纤维中研究较少的一种。从60年代发现至今,人们对动物细胞中间纤维的研究已经比较深入,近来又发现它在基因表达等重要生命活动中起一定的作用。中间纤维有一个显著的特征,就是能够在体外进行自我装配,不需要核苷酸和结合蛋白参加,也不依赖于蛋白质的浓度。植物细胞中是否存在中间纤维一直是未解决的问题。从80年代起,有一些研究发现在高等植物细胞中存在能与动物细胞中间纤维抗体进行

THE EFFECT OF SEVERAL FACTORS ON THE ASSEMBLY OF INTERMEDIATE FILAMENTS IN PLANT CELLS

We prepared the intermediate filament proteins from carrot suspension cells using sequential extraction, dissolved them in 9 mol/L urea, removed the pellet by ultra-centrifugation, after dialysis the supernatant, it can assemble into 10 nm filaments; different factors have different effect on their assembly in vitro: (1) pH8.0 is the favourite pH for the assembly of interma-diate filament in plant cells; (2) the IF proteins also can assemble in phosphate buffer, but the filament is not as smooth and compact as the normal one; (3) Mg2 and high ionic strength(150 mmol/L Nad) are good for the assembly of the filament, and also caused the aggregation of the filaments; (4) Ca2 often caused the filaments uncompleted,seldom caused the obvious aggregation of the filaments.