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Mist trickling bioreactor for <Emphasis Type="Italic">Centaurium erythraea</Emphasis> Rafn growth of shoots and production of secoiridoids |
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| Authors: | |
| Institution: | (1) Department of Pharmaceutical Biology and Biotechnology, Medical University, Muszynskiego Str. 1, 90–151 Lodz, Poland;(2) Present address: Department of Biology and Pharmaceutical Botany, Medical University, Kochanowskiego Str. 10, 51-601 Wroclaw, Poland |
| Abstract: | Shoots of Centaurium erythraea Rafn were cultivated in 5 l mist trickling bioreactor for 21 and 28 days increasing their dry weight from 0.54 g to 13.7 g and 18.3 g, respectively. About 6880 shoots from 223 initial shoot-tips in 21-day bioreactor producing cycle were produced. The shoots could be successfully rooted and transferred to soil. Secoiridoid accumulation (expressed as a sum of gentiopicroside, sweroside and swertiamarin) in shoots after 21 days of culture reached about 303 mg l−1. |
| Keywords: | Centaurium erythraea Rafn micropropagation mist trickling bioreactor secoiridoid glucosides shoot culture |
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