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Paclitaxel administration and its effects on clinically relevant human cancer and non cancer cell lines
Authors:Gretchen McAuliffe  Louis Roberts  Susan Roberts
Institution:(1) Department of Chemical Engineering, University of Massachusetts, Amherst, MA 01003, USA;(2) Department of Biology, University of Massachusetts, Amherst, MA 01003, USA
Abstract:Comparisons of the effects of clinically relevant concentrations of the anticancer agent paclitaxel on growth, viability, and apoptosis were determined using in vitro human cell cultures. Growth of the cervical cancer cell line, HeLa-S3, was significantly reduced, and apoptotic index was significantly increased, after 24 h in cultures treated with 12 nM paclitaxel. In contrast, hepatic carcinoma (HEpG2) cells capable of detoxifying paclitaxel were only affected at paclitaxel concentrations ge120 nM. The previously uncharacterized non-cancerous human microvessel endothelial cell line HMEC-1, was more sensitive to paclitaxel treatment than both HeLa-S3 and HEpG2 cells, demonstrating decreased growth and increased apoptosis with 1.2 nM paclitaxel. These results are significant in the design of in vitro cell culture systems to study drug metabolism and toxicity.
Keywords:apoptosis  cancer cell lines  HeLa-S3 cell line  HEpG2 cell line  HMEC-1 cell line  paclitaxel
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