Temporal Notch activation through Notch1a and Notch3 is required for maintaining zebrafish rhombomere boundaries |
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Authors: | Xuehui Qiu Chiaw-Hwee Lim Steven Hao-Kee Ho Kian-Hong Lee Yun-Jin Jiang |
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Institution: | (1) Laboratory of Developmental Signalling and Patterning, Genes and Development Division, Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), 61 Biopolis Drive, Singapore, 138673, Singapore;(2) Department of Biochemistry, National University of Singapore, 8 Medical Drive, Singapore, 117597, Singapore;(3) School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore; |
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Abstract: | In vertebrates, hindbrain is subdivided into seven segments termed rhombomeres and the interface between each rhombomere forms
the boundary. Similar to the D/V boundary formation in Drosophila, Notch activation has been shown to regulate the segregation of rhombomere boundary cells. Here we further explored the function
of Notch signaling in the formation of rhombomere boundaries. By using bodipy ceramide cell-labeling technique, we found that
the hindbrain boundary is formed initially in mib mutants but lost after 24 hours post-fertilization (hpf). This phenotype was more severe in mib
ta52b
allele than in mib
tfi91
allele. Similarly, injection of su(h)-MO led to boundary defects in a dosage-dependent manner. Boundary cells were recovered in mib
ta52b
mutants in the hdac1-deficient background, where neurogenesis is inhibited. Furthermore, boundary cells lost sensitivity to reduced Notch activation
from 15 somite stage onwards. We also showed that knockdown of notch3 function in notch1a mutants leads to the loss of rhombomere boundary cells and causes neuronal hyperplasia, indicating that Notch1a and Notch3
play a redundant role in the maintenance of rhombomere boundary. |
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Keywords: | |
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