Abstract: | Trichoderma harzianum produced RNA-depolymerase with maximum activity after 72 to 120 h of growth. Addition of K2HPO4 repressed enzyme production by the fungus. The optimal activity was at pH 7.8 and 40 to 50°C. The enzyme was stable at pH 3.2 to 9.0 and 80% of activity remained after 60 min at 40°C. EDTA and p-chloromercuribenzoate had no effect on the enzyme activity.E.S. Vasileva-Tonkova is with the Institute of Microbiology, Bulgarian Academy of Sciences, Acad, G. Bonchev str., B1. 26, 1113 Sofia, Bulgaria |