Alteration of collagenous protein profile in congestive heart failure secondary to myocardial infarction |
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Authors: | Vaclav Pelouch Ian M. C. Dixon Rajat Sethi Naranjan S. Dhalla |
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Affiliation: | (1) Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, 351 Tache Avenue, R2H 2A6 Winnipeg, Manitoba, Canada;(2) Faculty of Medicine, University of Manitoba, 351 Tache Avenue, R2H 2A6 Winnipeg, Manitoba, Canada |
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Abstract: | The rat model of myocardial infarction is characterized by progressive cardiac hypertrophy and failure. Rats with infarcts greater than 30% of the left ventricle exhibited early and moderate, stages of heart failure 4 and 8 weeks after the occlusion of the left coronary artery, respectively. As heart failure is usually associated with remodeling of the extracellular matrix, a histological and biochemical study of cardiac collagenous proteins was carried out using failing hearts. Total collagen content in the right ventricle increased at 2, 4, and 8 weeks following occlusion of the left coronary artery whereas such a change in viable left ventricle was seen after 4 and 8 weeks. Total cardiac hydroxyproline concentration was increased in both right and left ventricular samples from the infarcted animals when compared to those of control; this increase was due to elevation of pepsin-insoluble collagen fraction. The myocardial noncollagenous/collagenous protein ratio was decreased in experimental right and left ventricular samples when compared to control samples. These findings suggest that an increase in cross-linking of cardiac collagen as well as disparate synthesis of collagenous and noncollagenous proteins occurs in this model of congestive heart, failure. |
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Keywords: | collagen rat heart congestive heart failure myocardial infarction |
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