Quantitation of the accumulation of histone messenger RNA during oogenesis in Xenopus leavis |
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| Authors: | Walter van Dongen Rob Zaal Antoon Moorman Olivier Destrée |
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| Institution: | Department of Anatomy and Embryology, University of Amsterdam, Mauritskade 61, 1092 AD Amsterdam, The Netherlands |
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| Abstract: | The accumulation of messenger RNA coding for histone H3 in oogenesis of Xenopus laevis was studied by quantitative hybridization techniques, using a cloned genomic DNA fragment as a probe. This probe was isolated from cloned Xenopus histone DNA and contains most of the H3 coding sequences. Histone H3 mRNA accumulation was found to be completed before the maximum lampbrush stage. Hybridization of RNA blots with DNA probes containing genes for histones H2A, H2B, and H4 suggests the same accumulation pattern for the mRNAs coding for these histones as for histone H3 mRNA. The amount of H3 mRNA in the mature oocyte was established to be 130 ± 68 pg, i.e., about 5 × 108 copies. |
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