Rice anther culture: callus initiation and androclonal variation in progenies of regenerated plants |
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Authors: | P Sathish Oluf L Gamborg Murray W Nabors |
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Institution: | (1) Department of Biology, Colorado State University, 80523 Fort Collins, CO, USA;(2) Present address: Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, Stockholm University, 106 91 Stockholm, Sweden;(3) Present address: 1404 Solana Drive, 94002 Belmont, CA, USA |
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Abstract: | Summary Anthers from rice (Oryza sativa L.) subspecies japonica initiated more callus than their indica or indica x japonica counterparts. A mild stress, either by slow desiccation or heat shock, prior to the plating of anthers enhanced the ability to initiate callus. Slow dessication of anthers enhanced the ability of the japonica anthers to initiate callus even in medium that was supplemented with NaCl. The ability to initiate callus by the anthers plated on NaCl-supplemented medium decreased as the NaCl level in the medium increased. Among the regenerated plants 2.5% were albino and another 2% were haploid. Androclonal variation for tiller numbers, shoot height, plant dry matter and flowering were noticed in the progenies of the regenerated plants.Abbreviations NAA
-napthaleneacetic acid
- KT
Kinetin
- 2,4-D
2, 4-dichlorophenoxyacetic acid
- IAA
Indole-3-acetic acid
- BAP
N6 benzylaminopurine
- NFM
NaCl-free medium |
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