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Construction of a new catabolic pathway for d-fructose in Escherichia coli K12 using an l-sorbose-specific enzyme from Klebsiella pneumoniae |
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| Authors: | Birgitta M Wöhrl Georg A Sprenger Joseph W Lengeler |
| Institution: | (1) Max-Planck-Institut für Molekulare Genetik, Ihnestrasse 73, D-1000 Berlin 33, Federal Republic of Germany;(2) Institut für Biotechnologie 1, Kernforschungsanlage Jülich GmbH, Postfach 1913, D-5170 Jülich, Federal Republic of Germany;(3) Fachbereich Biologie/Chemie, AG Genetik, Universität Osnabrück, Postfach 4469, D-4500 Osnabrück, Federal Republic of Germany |
| Abstract: | Starting with a fruK (formerly fpk) mutant of Escherichia coli K12 lacking d-fructose-1-phosphate kinase (E.C. 2.7.1.3.), fructose positive derivatives were isolated after introduction of the cloned gene sorE from Klebsiella pneumoniae coding for an l-sorbose-1-phosphate reductase. The new pathway was shwon to proceed from d-fructose via d-fructose-1-phosphate and d-mannitol-1-phosphate to d-fructose 6-phosphate. It involves a transport system and enzymes encoded in the fru and the mtl operons from E. coli K12 as well as in the sor operon from K. pneumoniae respectively. |
| Keywords: | d-Fructose" target="_blank">d-Fructose New pathway l-Sorbose" target="_blank">l-Sorbose E coli K pneumoniae |
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