Dictyostelium discoideum chemotaxis: threshold for directed motion |
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Authors: | Song Loling Nadkarni Sharvari M Bödeker Hendrik U Beta Carsten Bae Albert Franck Carl Rappel Wouter-Jan Loomis William F Bodenschatz Eberhard |
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Institution: | Laboratory of Atomic and Solid State Physics, Department of Physics, Cornell University, Ithaca, NY 14853, USA. |
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Abstract: | The chemotactic response of Dictyostelium discoideum cells to stationary, linear gradients of cyclic adenosine 3',5'-monophosphate (cAMP) was studied using microfluidic devices. In shallow gradients of less than 10(-3) nM/microm, the cells showed no directional response and exhibited a constant basal motility. In steeper gradients, cells moved up the gradient on average. The chemotactic speed and the motility increased with increasing steepness up to a plateau at around 10(-1) nM/microm. In very steep gradients, above 10 nM/microm, the cells lost directionality and the motility returned to the sub-threshold level. In the regime of optimal response the difference in receptor occupancy at the front and back of the cell is estimated to be only about 100 molecules. |
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