含P301L突变的Tau转基因小鼠纯合子品系的建立及鉴定

目的:建立含P301L突变的tau转基因小鼠的纯合子品系。方法:雄原核显微注射法获得含P301L突变的tau转基因阳性首建鼠,通过SYBR Green实时荧光定量PCR法和传统育种方式结合鉴定纯合子和杂合子。结果:共选育出95只纯合子,鉴定出的纯合子具有优于杂合子模拟老年痴呆生物学特性改变的优势。结论:外源性基因tau能稳定遗传,采用的SYBR Green实时荧光定量PCR和传统育种方式结合筛选鉴定纯合子和杂合子快速、经济、可靠。

Breeding of transgenic mice expressing human tau isoform with P301L mutation and identification of homozygous transgenic mice

Objective: To establish homozygous transgenic mouse strain expressing human tau isoform with P301L mutation.Methods: Five transgenic mice expressing human tau isoform with P301L mutation were obtained by microinjection into male nuclei.Homozygote and hemizygote were identified by PCR and real-time fluorescent quantitative PCR.Results: Ninty five homozygous transgenic mice were selected,and the results indicateed that homozygous transgenic mice were superior to hemizygote in simulating the changes of biological characteristics.Conclusion: Exogenous gene tau is able to stably transmit to next generation and the combination of SYBR Green real-time fluorescent quantitative PCR with the traditional mating is a fast,reliable and economical way to screen homozygous and hemizygous transgenic mice.