大鼠肢体缺血/再灌注后多器官水肿及丹参的预防作用

目的:探讨大鼠肢体缺血/再灌注(LI/R)导致的多器官水肿及丹参的防治作用。方法:Wistar大鼠24只随机分为3组(n=8):对照组(C组)、缺血/再灌注组(I/R组)和丹参预处理组(SM组)。以止血带法制作大鼠肢体缺血/再灌注模型,SM组在再灌注前30 min经尾静脉推注丹参注射液5 ml/kg。准确留取每只动物的心、肝、肾、肺、脑、肠及骨骼肌组织各1 g,恒温烘干后称其干重并计算各组织的湿干重比值(W/D)。采用ELISA法测定血清白细胞介素1(IL-1)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNFα-)含量;采用生物化学方法测定超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。光镜下观察骨骼肌组织的形态学变化。结果:LI/R后各组织W/D均增加(P<0.05,P<0.01),血浆SOD活性降低而MDA含量增加(P<0.05,P<0.01),血清IL-1、IL-6、TNFα-水平均升高(P<0.05,P<0.01),骨骼肌组织镜下可见炎细胞浸润、肌纤维间隙增宽等病理改变。而SM组与单纯再灌注组比较,血清炎症因子水平下降,氧化损伤程度减轻,镜下组织形态学变化有所改善。结论:大鼠肢体缺血/再灌注可导致多器官水肿,丹参可通过抑制炎症反应、抗氧化等途径在一定程度上预防肢体缺血/再灌注后多器官的水肿。

Preventive effects of salvia miltiorrhiza on multiple organ edema in the rats of limb ischemia/reperfusion

Objective: To investigate the preventive effects of Salvia miltiorrhiza(SM) on multiple organ edema in the rats which suffered from hind limb ischemia/reperfusion(LI/R).methods: Twenty four Wistar rats were randomly divided into 3 groups(n=8): control group(C group),ischemia/reperfusion group(I/R group),Salvia miltiorrhiza group(SM group).Referring to Tourniquet method,the model rats which underwent 4 hours ischemia and 4 hours reperfusion of hind limbs were made.Thirty minutes before reperfusion,SM was given to the rats in SM group by tail vein injection at the dose of 5 ml/kg.Accurately weighed one gram of heart,liver, kidney,lung,brain,intestine and skeletal muscle from every animals,weigh these specimens after baking(60℃,55 hours),calculated the ratio of wet and dry(Wet/Dry,W/D).The levels of interleukin-1(IL-1)、interleukin-6(IL-6)、 tumor necrosis factor-α(TNF-α) in plasma and the contents of Superoxide dismutase(SOD) and malonaldehyde(MDA) were measured.The morphologic changes of skeletal muscle were observed with microscope.Results: It was found that after suffering from ischemia/reperfusion,the W/D of every specimens increased in different degree(P<0.05,P<0.01).In plasma,the values of SOD decreased but MDA increased obviously(P<0.05,P<0.01).The levels of IL-1、IL-6、TNF-α in plasma increased(P<0.05,P<0.01).After LI/R,infiltration of inflammatory cells,broaden interstitial around muscle fiber and disordered arrangement of muscle fibers could be seen under microscope.However,Compared with I/R group,W/D and levels of serum inflammatory factors in SM group were all lower,the values of SOD in plasma increased but MDA in plasma falled down.Pathological changes in skeletal muscle were improved.Conclusion: Limb ischemia/reperfusion can lead to multiple organ edema,Salvia miltiorrhiza can prevent the edema in some degree by anti-oxidation and anti-inflammation.