Elevations of intracellular cAMP result in a change in cell shape that resembles dome formation in cultured rat glomerular epithelial cells |
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Authors: | J I Kreisberg P Y Patel M A Venkatachalam G Taylor |
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Institution: | (1) Department of Pathology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, 78284 San Antonio, Texas |
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Abstract: | Summary Cultured glomerular epithelial cells form a continuous monolayer of polyhedral-shaped cells. PGE2 (1 μg/ml) in the presence of the phosphodiesterase inhibitor isobutylmethylxanthine (MIX) markedly raises intracellular and
medium cyclic AMP (cAMP) levels at 20 min (intracellular: MIX alone, 112 ± 6.6 pmol cAMP/mg protein, MIX plus PGE2, 2252±63 pmol cAMP/mg protein; medium: MIX, 20.6±2.1 pmol cAMP/mg protein; MIX plus PGE2, 117±3.8 pmol cAMP/mg protein). By 2 h, when cellular and medium cAMP levels were still elevated, the cells underwent a change
in shape that was similar to dome formation (15 to 20% of the monolayer changing shape). Derivatives of cAMP i. e. dibutyryl
and 8-(4-chlorophenylthio)-cAMP], when added to the incubation medium also caused shape change in glomerular epithelial cells
at 2 h; cAMP itself did not. The formation of domes has been used as a morphological indicator of the vertorial transport
of salt and water in other cultured epithelial cells.
This work was supported by grant AM 29787 from the National Institutes of Health, Bethesda, MD. |
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Keywords: | culture glomerular epithelial cells cAMP dome formation |
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