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Isolation of the first toxin from the scorpion Buthus occitanus israelis showing preference for Shaker potassium channels |
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| Authors: | Kozminsky-Atias Adi Somech Erez Zilberberg Noam |
| Institution: | Department of Life Sciences and the Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel. |
| Abstract: | We have purified BoiTx1, the first toxin from the venom of the Israeli scorpion, Buthus occitanus israelis, and studied its activity and genomic organization. BoiTx1 is a 37 amino acid-long peptide contained six conserved cysteines, and is classified as an alpha-KTx3.10 toxin. The pharmacological effects of BoiTx1 were studied on various cloned K(+) channels expressed in Xenopus laevis oocytes. BoiTx1 inhibited currents through Drosophila Shaker channels with an IC(50) value of 3.5+/-0.5nM, yet had much lesser effect on its mammalian orthologs. Thus, BoiTx1 is the first member of the alpha-KTx3 family that preferentially affects insect potassium channels. |
| Keywords: | MALDI-TOF matrix-assisted laser desorption ionization-time-of-flight HPLC high performance liquid chromatography KTx K+ channel toxin Kv voltage-gated K+ channel TFA trifluoroacetic acid DTT dithiothreitol TEVC two-electrode voltage clamp technique BSA bovine serum albumin |
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