Respiratory deficient mutants of Rhodopseudomonas capsulata |
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Authors: | Hendrik Hüdig Norbert Kaufmann Gerhart Drews |
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Affiliation: | (1) Institute of Biology II, Microbiology, Albert-Ludwigs-University, Schänzlestr. 1, D-7800 Freiburg, Federal Republic of Germany |
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Abstract: | The facultative photosynthetic bacterium Rhodopseudomonas capsulata was mutagenized by transfer of the plasmid pSUP201::Tn5 from Escherichia coli to R. capsulata. Mutants defective in cytochrome oxidase and other respiratory functions were selected by replica plating, NADI-reaction and immunological methods. Among 20,000 mutants no clone was detected, which lacks the 65,000-protein of the cytochrome oxidase, but many mutants have been isolated which were cytochrome oxidase deficient (or inactive). Other mutants excrete heme and cytochrome c into the medium and lack cytochrome c2.Abbreviations Ap ampicillin - CIE crossed immunoelectrophoresis - cyt cytochrome - Cm chloramphenicol - Km kanamycin - SDS sodium dodecylsulfate - Tc tetracycline |
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Keywords: | Transposon Tn5 Cytochrome oxidase Cytochrome c Respiratory mutants Rhodopseudomonas capsulata |
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