Autoinduction of a genetic locus encoding putative acyltransferase in <Emphasis Type="Italic">Corynebacterium glutamicum</Emphasis> |
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Authors: | Hee-Sung Shin Yong-Jae Kim In-Hwa Yoo Heung-Shick Lee Shouguang Jin Un-Hwan Ha |
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Institution: | (1) Department of Biotechnology and Bioinformatics, Korea University, Yeongi, Chungnam, 339-700, Republic of Korea;(2) Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL 32610, USA; |
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Abstract: | A genetic locus, encoding putative acyltransferase, was induced by autoinducers in Corynebacterium glutamicum. The autoinducers were maximally produced by the bacterium after 24 h culture. Those molecules are resistant to proteinase
K treatment (300 μg ml−1) for 30 min at 37°C or at 121°C for 15 min, and remained stable after extensive storage at 4°C. Autoinducers in the cell-free
culture fluids from Corynebacterium ammoniagenes and Pseudomonas aeruginosa also induced the expression of acyltransferase in C. glutamicum, suggesting possible cross-recognition of the autoinducers by C. glutamicum. C. glutamicum thus possesses an autoinduction system which secretes autoinducers during growth, triggering the expression of downstream
genes, exemplified by the putative acyltransferase gene. |
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