Overexpression and simple purification of the Thermotoga maritima 6-phosphogluconate dehydrogenase in Escherichia coli and its application for NADPH regeneration |
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| Authors: | Yiran Wang and Y-H Percival Zhang |
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| Institution: | (1) Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, 210-A Seitz Hall, 24061 Virgina, USA;(2) Institute for Critical Technology and Applied Sciences (ICTAS), Virginia Polytechnic Institute and State University, Blacksburg, 24061 Virgina, USA;(3) DOE BioEnergy Science Center (BESC), Oak Ridge, 37831 Tennessee, USA |
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| Abstract: | Background Thermostable enzymes from thermophilic microorganisms are playing more and more important roles in molecular biology R&D and
industrial applications. However, over-production of recombinant soluble proteins from thermophilic microorganisms in mesophilic
hosts (e.g. E. coli) remains challenging sometimes. |
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