Gene expression, cellular localization, and enzymatic activity of diacylglycerol kinase isozymes in rat ovary and placenta |
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| Authors: | Mayumi Toya Yasukazu Hozumi Tsukasa Ito Morihiko Takeda Fumio Sakane Hideo Kanoh Hidekazu Saito Masahiko Hiroi Hirohisa Kurachi Hisatake Kondo Kaoru Goto |
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| Institution: | (1) Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Iida-nishi 2-2-2, Yamagata 990-9585, Japan;(2) Department of Obstetrics and Gynecology, Yamagata University School of Medicine, Iida-nishi 2-2-2, Yamagata 990-9585, Japan;(3) Department of Medicine, Tohoku University Graduate School of Medical Science, Seiryo-cho 2-1, Sendai 980-8575, Japan;(4) Department of Biochemistry, Sapporo Medical University School of Medicine, South-1, West-17, Sapporo 060-8556, Japan;(5) Department of Perinatal Medicine and Maternal Care, National Center for Child Health and Development, Okura 2-10-1, Setagaya-ku, Tokyo 157-8535, Japan;(6) Yamagata Prefectural University of Health Sciences, Kamiyanagi 210, Yamagata 990-2212, Japan;(7) Department of Cell Biology, Division of Histology, Tohoku University Graduate School of Medical Science, Seiryo-cho 2-1, Sendai 980-8575, Japan |
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| Abstract: | Female reproductive organs show remarkable cyclic changes in morphology and function in response to a combination of hormones. Evidence has accumulated suggesting that phosphoinositide turnover and the consequent diacylglycerol (DG) protein kinase C (PKC) pathway are intimately involved in these mechanisms. The present study has been performed to investigate the gene expression, cellular localization, and enzymatic activity of the DG kinase (DGK) isozymes that control the DG-PKC pathway. Gene expression for DGK , - , - , and - was detected in the ovary and placenta. Intense expression signals for DGK and - were observed in the theca cells and moderate signals in the interstitium and corpora lutea of the ovary. On the other hand, signals for DGK were seen more intensely in granulosa cells. In the placenta, signals for DGK and - were observed in the junctional zone, whereas those for DGK were detected in the labyrinthine zone. At higher magnification, the signals for DGK were mainly discerned in giant cytotrophoblasts, and those for DGK were found in small cytotrophoblasts of the junctional zone. DGK signals were observed in all cellular components of the labyrinthine zone, including mesenchyme, trabecular trophoblasts, and cytotrophoblasts. DGK signals were detected in the junctional zone on day 13 and 15 of pregnancy and were diffusely distributed both in the labyrinthine and junctional zones at later stages. The present study reveals distinct patterns of mRNA localization for DGK isozymes in the rat ovary and placenta, suggesting that each isozyme plays a unique role in distinct cell types in these organs.This work was supported by Grants-in-Aids from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan, the Uehara Memorial Foundation, the ONO Medical Research Foundation, the Ciba-Geigy Foundation (Japan) for the Promotion of Science, the Kato Memorial Bioscience Foundation, and the Yamagata Health Support Foundation (to K.G.). |
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| Keywords: | Diacylglycerol kinase Diacylglycerol protein kinase C pathway Ovary Placenta Subcellular localization Rat (Wistar female) |
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