| 赤霉菌原生质体DNA转化* |
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| 引用本文： | 朱平,杨金玲,朱慧新,程克棣.赤霉菌原生质体DNA转化*[J].菌物学报,2000,19(2). |
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| 作者姓名： | 朱平 杨金玲 朱慧新 程克棣 |
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| 作者单位： | 中国医学科学院 中国协和医科大学 药物研究所卫生部天然药物生物合成重点实验室 |
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| 摘 要： | Methods for the protoplast preparation and DNA transformation of Gibberella fujikuroi were established. The protoplasts were transformed with plasmid pAN7-1, which carries hygromycin B resistant gene (hPh), and the transformation frequency was about 10. Some transformants grew vigorously on the selectivemedium containing 400μg / ml of hygromycin B, while the minimum inhibitory concentration (MIC) of the antibiotic to the recipient strain was oniy 20μg / ml. Southern hybridization showed that the hph gene ha…
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| 关 键 词： | 赤霉菌 原生质体 DNA转化 潮霉素B抗性基因 |
DNA TRANSFORMATION OF GIBBERELLA FUJIKUROI PROTOPLAST |
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| Abstract: | Methods for the protoplast preparation and DNA transformation of Gibberella fujikuroi were established. The protoplasts were transformed with plasmid pAN7-1, which carries hygromycin B resistant gene (hPh), and the transformation frequency was about 10. Some transformants grew vigorously on the selectivemedium containing 400μg / ml of hygromycin B, while the minimum inhibitory concentration (MIC) of the antibiotic to the recipient strain was oniy 20μg / ml. Southern hybridization showed that the hph gene has integrated into the genomic DNA of the recipient strain in different manners. |
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