The fungicidal diamine (E)-(N,N,N',N'-tetraethyl)-1,4-diaminobut-2-ene increases spermine concentration in the late blight fungus Phytophthora infestans |
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Authors: | N.D. Havis,D.R. Walters,& D.J. Robins |
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Affiliation: | Department of Plant Science, The Scottish Agricultural College, Auchincruive, Ayr,;Department of Chemistry, University of Glasgow, Glasgow, UK |
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Abstract: | Mycelial growth, polyamine concentrations and the activities of enzymes of polyamine biosynthesis and catabolism were examined in the late blight fungus Phytophthora infestans , grown in the presence of the putrescine analogue (E)-( N , N , N ', N '-tetraethyl)-1,4-diaminobut-2-ene (E-TED). Growth of P. infestans was significantly reduced by 5 mmol l−1 E-TED but concentrations of 1 mmol l−1 or less had no effect. All concentrations of E-TED significantly increased the spermine concentrations in fungal tissue but these increases were not accompanied by any significant alterations in enzyme activities. An examination of the uptake of 14C-spermine into cells grown in the presence of E-TED indicated that spermine could be subject to replacement by E-TED at intracellular binding sites. E-TED-treated cells were shown to excrete spermine at significantly higher levels than untreated cells. Permeabilization of cells with n -butanol removed this difference. Possible causes for this difference are discussed. |
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