Partial purification of the glucocorticoid receptor from rat liver: a rapid, two-step procedure using DNA-cellulose. |
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| Authors: | H J Eisen W Glinsmann |
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| Affiliation: | Laboratory of Biomedical Sciences National Institute of Child Health and Human Development National Institutes of Health, Bethesda, Maryland 20014 USA |
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| Abstract: | Glucocorticoid receptor from rat liver was purified 1800-fold by a rapid two-step procedure using DNA-cellulose. The procedure is based on increasing the affinity of the glucocorticoid-receptor complex for DNA by heating the complex. During a first chromatography step, unheated glucocorticoid-receptor complex is separated from cytosol proteins that bind to DNA-cellulose with high affinity. During a second chromatographic step, heat-treated glucocorticoid-receptor complex is separated from proteins with low affinity for DNA. The partially purified complex is functionally competent in that it is taken up by isolated rat liver nuclei. |
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