|
|||||
|
|
Role of LSM34/SpSM50 proteins in endoskeletal spicule formation in sea urchin embryos |
|
| Authors: | Fred Wilt Lindsay Croker Christopher E Killian Kent McDonald |
| Institution: | Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3200, USA; School of Medicine, University of Maryland, Baltimore, Maryland 21201, USA; Electron Microscope Laboratory, University of California, Berkeley, California 94720-3330, USA |
| Abstract: | Abstract. Sea urchin embryos form an endoskeletal spicule composed of calcium carbonate and occluded matrix proteins. The accumulation of the LSM34 spicule matrix protein in embryos of Lytechinus pictus (and its ortholog, SpSM50, in Strongylocentrotus purpuratus ) has been inhibited using morpholino antisense oligonucleotides. The inhibition, using relatively high levels of antisense reagent, can result in the complete absence of spicules, and the complete loss of immunoreactive LSM34/SpSM50, as judged by immunostaining and Western blotting. Primary mesenchyme cells (PMCs) do form and express PMC-specific cell surface antigens despite this inhibition. However, these anti-LSM34/SpSM50-treated embryos do not accumulate SM30 protein, another major matrix protein. Hence, both the initiation of spicule formation and subsequent morphogenesis require LSM34 accumulation in L. pictus , and the accumulation of its ortholog, SpSM50, in S. purpuratus . |
| Keywords: | skeletal biomineralization |