Possible mechanisms in the rearrangement of non-yolk cytoplasmic materials during maturation of theXenopus laevis oocyte |
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Authors: | Akio S Suzuki Junichi Manabe Hiroshi Imoh |
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Institution: | (1) Department of Biology, Faculty of General Education, Kumamoto University, 860 Kumamoto, Japan;(2) Department of Biology, Faculty of Education, Miyazaki University, Miyazaki, Japan |
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Abstract: | Using the monoclonal antibody (MoAb) Xa5B6 as probe, the authors examined the mechanisms of cytoplasmic rearrangement occurring
during maturation of theXenopus oocyte. The antigen molecules recognized by the MoAb are arranged in radial striations of the oocyte cytoplasm. The radial
striations were disorganized in vitro by progesterone treatment, and the antigen molecules were uniformly distributed, predominantly
in the animal hemisphere. Even when the germinal vesicle was mechanically removed or when germinal vesicle breakdown was suppressed
in a K+-free medium, progesterone induced a disorganization of the radial striations. This progesterone-induced disorganization was
inhibited by the protein synthesis inhibitor cycloheximide. When full-sized oocytes were treated with cytochalasin B, the
radial striations were also disorganized, but the antigen molecules did not disperse into the large mass. Colchicine treatment
had little effect. Antigen molecules were no longer arranged in radial striations and were completely dispersed when the oocyte
was simultaneously treated with both drugs. These results indicate that the two compartments in the oocyte cytoplasm, the
yolk-free cytoplasm and yolk column, are organized by different types of cytoskeletal system. It is also suggested that the
maturation-promoting factor (MPF) activated during progesterone-induced maturation disrupts these cytoskeletal systems and
disorganizes the radial striations.
Correspondence to: A.S. Suzuki |
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Keywords: | Maturation Rearrangement Xenopus Maternal cytoplasm Cytoskeleton |
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