High-resolution autoradiography of nuclear modifications during and after heat treatment ofNeurospora crassa |
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Authors: | The Can Ton-That G. Turian |
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Affiliation: | (1) Laboratoire de Microbiologie Générale, Département de Biologie Végétale, Université de Genéve, 3, Place de l'Université, CH-1211 Genéve 4, Switzerland |
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Abstract: | Summary The appearance of perinucleolar electron-dense spots in the nuclei of macroconidia ofNeurospora crassa incubated at 46 C and their disaggregation after shift-down to 25 C have been investigated by high-resolution autoradiography after (5-3H) uridine pulses with or without chase periods. The RNA of these ribonucleoprotein-rich dense spots has been found to originate mainly from the heatsensitive nucleolus; after return to 25 C, the nucleolar activity was recovered and the RNA material stored either in an unprocessed or a mature rRNA form in the dense spots was found to be progressively extruded into the cytoplasm. |
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Keywords: | High-resolution autoradiography Neurospora Heat treatment Perinucleolar dense spots |
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