Improvement of Interfacial Protein Stability by CHAPS |
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Authors: | Hongkee Sah Kil-Soo Kim |
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Affiliation: | (1) College of Pharmacy, Ewha Womans University, 11-1 Daehyun-Dong, Seodaemun-Gu, Seoul, 125-720, Korea |
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Abstract: | Emulsification of aqueous protein solutions in methylene chloride triggered the formation of water-insoluble aggregates at a water/methylene chloride interface. As a result, the amounts of β-lactoglobulin and ovalbumin recovered in water were 36 and 44%, respectively. Addition of 5 mm CHAPS in the aqueous phase raised the degree of β-lactoglobulin recovery to 96%. Sodium taurocholate, however, failed to improve protein recovery. The stabilizing effect of CHAPS was also protein-specific and concentration-dependent: at ≥5 mm, the surfactant caused unfolding of ovalbumin to make a water-soluble oligomer. CHAPS thus stabilizes proteins at an interface. Revisions requested 24 November 2005; Revisions received 17 January 2006 |
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Keywords: | CHAPS interface microspheres protein stability surfactants |
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