Ptch1-mediated dosage-dependent action of Shh signaling regulates neural progenitor development at late gestational stages |
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Authors: | Shikata Yayoi Okada Toshiaki Hashimoto Mitsuhiro Ellis Tammy Matsumaru Daisuke Shiroishi Toshihiko Ogawa Masaharu Wainwright Brandon Motoyama Jun |
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Affiliation: | aBrain Science Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan;bInstitute for Molecular Bioscience, Queensland Bioscience Precinct, 306 Carmody Road, The University of Queensland, St Lucia 4072, Australia;cCenter for Animal Resources and Development (CARD), Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 860-0811, Japan;dMammalian Genetics Laboratory, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan;eDepartment of Medical Life Systems, Doshisha University, 4-1-1 Kizukawadai, Kizukawashi, Kyoto 619-0225, Japan |
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Abstract: | Sonic hedgehog (Shh) signaling regulates cell differentiation and proliferation during brain development. However, the role of Shh in neurogenesis during late gestation (embryonic day 13.5–18.5) remains unclear. Herein, we used a genetic approach and in utero electroporation to investigate the role of mouse Shh and patched homolog 1 (Ptch1), the putative receptor for Shh. Proliferating cortical intermediate (basal) progenitor cells (IPCs) were severely reduced in Shh mutant mice, suggesting that endogenous Shh signaling could play an essential role in cortical IPC development. During cortical neurogenesis, strong upregulation of Shh signaling enhanced the transition from ventricular zone (VZ) progenitors to ventralized IPCs, while low levels of signaling enhanced the generation and proliferation of cortical IPCs in the subventricular zone. The effects of Shh upregulation in this study were consistent with a phenotype of conditional loss of function of Ptch1, and the phenotype of a hypomorphic allele of Ptch1, respectively. These data indicated that endogenous Ptch1 mediates the broad effects of Shh on the transition from VZ progenitors to IPCs and activation of proliferation of the IPCs in the cortex during late gestational stages. |
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Keywords: | Neural progenitor cell Differentiation Proliferation Neurogenesis Sonic hedgehog |
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