Protogenin mediates cell adhesion for ingression and re-epithelialization of paraxial mesodermal cells |
| |
Authors: | Ito Kodai Nakamura Harukazu Watanabe Yuji |
| |
Institution: | aDepartment of Molecular Neurobiology, Graduate School of Life Sciences, Tohoku University, 4-1 Seiryo-machi. Aoba-ku, Sendai 980-8575, Japan;bInstitute of Development, Aging and Cancer, Tohoku University, Japan |
| |
Abstract: | In the avian embryo, precursor cells of the paraxial mesoderm that reside in the epiblast ingress through the primitive streak and migrate bilaterally in an anterolateral direction. Herein, we report on the roles of Protogenin (PRTG), an immunoglobulin superfamily protein expressed on the surface of the ingressing and migrating cells that give rise to the paraxial mesoderm, in paraxial mesoderm development. An aggregation assay using L-cells showed that PRTG mediates homophilic cell adhesion. Overexpression of PRTG in the presumptive paraxial mesoderm delayed mesodermal cell migration due to augmented adhesiveness. In contrast, siRNA knockdown of PRTG impaired successive ingression of epiblast cells and disorganized the epithelial structure of the somites. These results suggest that PRTG mediates cell adhesion to regulate continuous ingression of cells giving rise to the paraxial mesodermal lineage, as well as tissue integrity. |
| |
Keywords: | Ingression Migration Paraxial mesoderm Adhesion Chick |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|