Repair of potentially lethal damage in yeast induced by fast neutrons |
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Authors: | M A Hannan M Paul D P Gibson F A Al-Watban |
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Affiliation: | Biological Research Department, Research Centre, King Faisal Specialist Hospital, Box 3354, Riyadh 11211 Kingdom of Saudi Arabia Saudi Arabia |
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Abstract: | Survival curves of 3 diploid (D7) yeast strains: one wild-type, one deficient in excision of pyrimidine dimers (UV-sensitive) and one blocked in DNA double-strand-break repair (X-ray-sensitive), were compared after irradiation with cyclotron-produced fast neutrons. It was observed that both the UV-sensitive (rad3/rad3) and the X-ray-sensitive (rad52/rad52) mutants were more sensitive to neutrons than the wild-type. The role of DNA double-strand-breaks in neutron-induced cell death was further studied by comparing the relative sensitivity of the rad52/rad52 mutant to gamma-rays and fast neutrons. A comparison of the dose modification factors revealed that the deficiency in DNA double-strand-break repair did not make the yeast cells more sensitive to neutrons than to photons, which suggests that lesions of a different type may also be produced by neutrons. Survival curves obtained upon immediate plating and after delayed plating of neutron-irradiated cells showed that all 3 yeast strains were efficient in liquid holding recovery. The role of different repair pathways in cellular recovery from neutron-induced lethal damage is discussed. |
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Keywords: | Correspondence and reprint requests: Mohammed A. Hannah Ph.D. Biological Research Department Research Centre King Falsal Specialist Hospital P.O. Box 3354 Riyadh 11211 (Kingdom of Saudi Arabia). |
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