Dissociation of human interferon-gamma-like activity from migration-inhibition factor |
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Authors: | Weishui Y Weiser Heinz G Remold Lutz H Block John R David |
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Institution: | 1. Department of Medicine, Harvard Medical School, Boston, Massachusetts, U.S.A.;2. Department of Rheumatology and Immunology, Brigham and Women''s Hospital, Boston, Massachusetts 02115 U.S.A.;3. Medizinische Poliklinik der Universitat Munchen, Munchen, Federal Republic of Germany |
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Abstract: | Supernatants harvested from concanavalin A-stimulated human peripheral mononuclear cells after 24 hr of incubation contain one interferon species similar to human interferon-gamma (IFN-γ) with a pI of 4.6–5.3 (first day pH 5 IFN-γ). In contrast, during the subsequent 24 hr of incubation two species with properties of IFN-γ are produced with pI of 3.6–4.0 (second day pH 4 IFN-γ) and 4.6–5.6 (second day pH 5 IFN-γ), respectively. First day pH 5 IFN-γ and second day pH 5 IFN-γ have been found to differ on the basis of trypsin sensitivity. This pattern of polymorphism is similar to the pattern previously described for human migration-inhibitory factor (MIF) which can be separated into first day pH 5 MIF, second day pH 3 MIF, and second day pH 5 MIF. However, IFN-γ-like species can be differentiated from MIF biochemically and antigenically. Fractions with second day pH 4 IFN-γ have no MIF activity and fractions with second day pH 3 MIF contain no IFN activity. In addition, first and second day pH 5 MIF, which also contain IFN-γ activity, can be separated from the latter by precipitation as well as neutralization with polyclonal and monoclonal anti-human MIF antibodies. |
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Keywords: | To whom correspondence should be sent at: Department of Rheumatology and Immunology Seeley G Mudd Building 250 Longwood Avenue Boston Mass 02115 |
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