Long-lived IgE- and IgG-secreting cells in rodents manifesting persistent antibody responses |
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Authors: | PG Holt JD Sedgwick C OLeary K Krska S Leivers |
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Institution: | 1. Clinical Immunology Research Unit, Princess Margaret Children''s Medical Research Foundation, Princess Margaret Hospital, Subiaco 6008, Australia;2. Department of Microbiology, University of Western Australia, Perth, Western Australia, Australia |
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Abstract: | BALB/c mice and BN rats manifesting persistent IgE and IgG responses were examined up to 1 year after immunization. A significant proportion of the ongoing antibody response in these animals survived lethal X-irradiation employing dosages sufficient to deplete B memory cells. The persistent IgE responses in both species were refractory to exogenous isotype-specific suppressor cells taken from tolerant syngeneic animals, which were shown to abrogate primary IgE responses in parallel tests. Employing a novel ELISA-based assay for plaque forming cells, long-lived radioresistant IgE- and IgG-secreting cells were identified in differing ratios in lymph nodes, spleen, and bone marrow of both species. These long-lived cells were shown to arise following maximum antigenic challenge with antigen plus adjuvant, and after repeated low-grade stimulation by antigen alone, including passive inhalation of dilute antigen aerosols. |
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Keywords: | To whom correspondence should be addressed |
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