Heterogeneous accessory cell requirement for human peripheral blood T lymphocyte activation by PHA into IL-2-responsive colony-forming cells |
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Authors: | Jean Pierre Farcet Noufissa Oudhriri Marie-Françoise Gourdin Jacqueline Bouguet Didier Fradelizi Félix Reyes |
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Institution: | 1. INSERM U.91, Hôpital Henri Mondor, 94010 Creteil, France;2. Laboratoire des Interactions Lymphocytaires, Institut de Recherches sur les Maladies du Sang, Hôpital Saint Louis, 75475 Paris Cedex 10, France |
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Abstract: | Mitogen-driven T cell proliferation in liquid culture requires accessory cells that cooperate in interleukin 2 production. We have investigated the accessory cell requirement for human lymphocyte colony formation under PHA stimulation. Semisolid medium limits cell-to-cell contact emphasizing the role of cooperating cells both in growth factor production and in triggering events. Culturing at high T cell density demonstrates that accessory cells can be substituted for colony formation by exogenous IL-2. Culturing at low T cell density in the presence of IL-2 also demonstrates that accessory cells are required for activation of a subset of progenitors into IL-2 responsive colony-forming cells. Consequently, T colony progenitors, contained in the E-rosetting cell fraction of peripheral blood, are heterogeneous in their triggering signals: a minor subset is directly inducible by PHA, and a major subset is inducible by PHA in the presence of accessory cells. We found that monocytes and some leukemic B cells support effective accessory function in both colony growth factor production and colony progenitor sensitization. |
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