Anaerobic degradation of hydroaromatic compounds by newly isolated fermenting bacteria |
| |
Authors: | Andreas Brune Berhard Schink |
| |
Affiliation: | (1) Lehrstuhl Mikrobiologie I, Universität Tübingen, Auf der Morgenstelle 28, W-7400 Tübingen, Germany;(2) Present address: Department of Microbiology and Public Health, Michigan State University, 48824-1101 East Lansing, MI, USA;(3) Present address: Fakultät für Biologie, Universität Konstanz, Postach 5560, W-7750 Konstanz, FRG |
| |
Abstract: | Aerobic organisms degrade hydroaromatic compounds via the hydroaromatic pathway yielding protocatechuic acid which is further metabolized by oxygenase-mediated ring fission in the 3-oxoadipate pathway. No information exists on anaerobic degradation of hydroaromatics so far. We enriched and isolated from various sources of anoxic sediments several strains of rapidly growing gram-negative bacteria fermenting quinic (1,3,4,5-tetrahydroxy-cyclohexane-1-carboxylic acid) and shikimic acid (3,4,5-trihydroxy-1-cyclohexene-1-carboxylic acid) in the absence of external electron acceptors. Quinic and shikimic acid were the only ones utilized of more than 30 substrates tested. The marine isolates formed acetate, butyrate, and H2, whereas all freshwater strains formed acetate and propionate as typical fermentation products. Aromatic intermediates were not involved in this degradation. Characterization of the isolates, fermentation balances for both hydroaromatic compounds, and enzyme activities involved in one degradation pathway are presented.Abbreviations BV benzyl viologen (1,1 -dibenzyl-4,4 -bipyridinium dichloride) - CoA coenzyme A - CTAB cetyltrimethylammonium bronide - DCPIP 2,4-dichlorophenolindophenol - DTT 1,4-dithiotheriol - MV methyl viologen (1,1 -dimethyl-4,4 -bipyridinium dichloride) - Tricine N-[tris-(hydroxymethyl)-methyl]-glycine - Tris tris-(hydroxymethyl)-aminomethane |
| |
Keywords: | Anaerobic degradation Hydroaromatic compounds Quinic acid Shikimic acid |
本文献已被 SpringerLink 等数据库收录! |
|