Control of autogenous activation of Herbaspirillum seropedicae nifA promoter by the IHF protein |
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Authors: | Wassem Roseli Pedrosa Fábio O Yates Marshall G Rego Fabiane G M Chubatsu Leda S Rigo Liu U Souza Emanuel M |
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Institution: | Departamento de Bioquímica - UFPR, Caixa Postal 19046, CEP 81531-990, Curitiba, PR Brazil. |
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Abstract: | Analysis of the expression of the Herbaspirillum seropedicae nifA promoter in Escherichia coli and Herbaspirillum seropedicae, showed that nifA expression is primarily dependent on NtrC but also required NifA for maximal expression under nitrogen-fixing conditions. Deletion of the IHF (integration host factor)-binding site produced a promoter with two-fold higher activity than the native promoter in the H. seropedicae wild-type strain but not in a nifA strain, indicating that IHF controls NifA auto-activation. IHF is apparently required to prevent overexpression of the NifA protein via auto-activation under nitrogen-fixing conditions in H. seropedicae. |
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Keywords: | NifA Nitrogen fixation Integration host factor Herbaspirillum |
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