| 尿素改善SDS-PAGE分离小分子肽的效果 |
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| 引用本文： | 曹佐武. 尿素改善SDS-PAGE分离小分子肽的效果[J]. 生物技术, 2003, 13(5): 23-24 |
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| 作者姓名： | 曹佐武 |
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| 作者单位： | 暨南大学生殖免疫研究中心,广东,广州,510632 |
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| 基金项目： | 国家自然科学基金项目 (No.30 2 71 370 )，广东省自然科学基金项目 (No.0 1 0 398)资助 |
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| 摘 要： | 目的：探讨尿素对Tricine—SDS—PAGE系统分离小分子蛋白的影响。方法：利用常规SDS-PAGE和Tricine-SDS-PAGE分离小分子肽，比较不同组成的分离胶的分离效果。结果：调整聚丙烯酰胺凝胶的分子组成，使丙烯酰胺和甲叉双丙烯酰胺的交联度为5．05％，能够改善小分子肽的分离效果。加入36％的尿素比加入甘油可以更有效分离1kD的小肽。但尿素胶聚合太快影响分离效果。结论：尿素改善SDS—PAGE分离小分子肽的效果，制作尿素胶时，宜采用低浓度的AP和TEMED使凝胶慢速聚合。
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| 关 键 词： | 尿素 SDS—PAGE 分离 肽 聚丙烯酰胺凝胶电泳 |
| 文章编号： | 1004-311X(2003)05-0023-02 |
| 修稿时间： | 2003-04-16 |
Urea Improves the SDS-PAGE Results of Low Molecular Peptides |
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| CAO Zuo-wu. Urea Improves the SDS-PAGE Results of Low Molecular Peptides[J]. Biotechnology, 2003, 13(5): 23-24 |
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| Authors: | CAO Zuo-wu |
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| Abstract: | Objective:To investigate the effect of urea on the resolution of the low molecular peptides by the Tricine-SDS-PAGE.Methods:Small molecular peptides were analyzed by both regular SDS-PAGE and Tricine-SDS-PAGE.Different resolving gels were compared.Results:The resolving gel with 5.05% cross-link of acrylamide to bisacrylamide can separate the protein smaller than 3 kD.The addition of 36% urea is better than glycerol to the resolution of 1kD peptide.Conclusions:Urea is an effective factor to the resolution of 1kD peptide slow polymerization with low content of AP and TEMED was beneficial. |
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| Keywords: | SDS-PAGE low molecular peptide urea |
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