Inhibitory effects of pertussis toxin on a depolarization-evoked Ca2+ influx in NG108-15 cells |
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Authors: | Y Nomura M Tohda |
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Affiliation: | Department of Pharmacology, Research Institute of Wakan-Yaku (Oriental Medicine), Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-01, Japan |
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Abstract: | Depolarized stimulation 1.5-fold increased Ca2+ influx which was inhibited by pretreatment with verapamil or LaCl3. Treatment with pertussis toxin, islet-activating protein (IAP), induced a reduction in 50 mM K+-induced Ca2+ influx and stimulated adenylate cyclase (AC) activity in NG108-15 cells. However, addition of dibutyryl cAMP or forskolin treatment elevating cAMP level exerted no effects on a depolarization-induced Ca2+ influx. Dissociated B-oligomer of IAP after treatment with dithiothreitol and ATP increased a depolarization-evoked Ca2+ influx. It is suggested that inhibitory GTP-binding protein (G1) or other IAP substrate proteins could directly be involved in Ca2+ influx via voltage-sensitive Ca2+ channel. |
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